Distinguishing feature:

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• Really easy to implement

• High flexibility

• A unique amplification and revelation which is the same for every test

• Small packaging

• Visual reading

• Easy interpretation

• Integrated inspections

• Possible automation

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Summary: The DNA from the sample (bacterial colonies, biopsy, blood) is isolated thanks to GenoLyse® reagent (approximately 10 minutes) then amplified by PCR and quickly detected on a strip via hybridization followed by chromogenic development.
 

Quick technologie for maximum sensitivity and specificity.
 

Stage 1. Biotinylated primers enable to amplify the DNA. By this way, biotin molecules are incorporated to the amplicons.

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Stage 2. Amplicons are denaturated and incubated in the presence of a strip which is covered with specific measuring tubes complementary to amplified nucleic acids sequences.

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Stage 3. Amplicons can form hybrids which are specific to each primer fixed to the strip or are eliminated during each washing if the sequence does not match.

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Stage 4. Streptavidin conjugated with alkaline phosphatase is fixed to biotin contained in amplicons immobilized on the strip by creating a Biotin - Streptavidin - Alkalin Phosphatase complex.

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Stage 5. A colourless chromogenic substrat precipitates under the action of alkalin phosphaste by provoking the
development of one or several coloured strips.

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Stage 6. The interpretation of obtained strip profiles enables to identify a type or a particular gene.

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Internal check:

Each strip contains two controls: 

•  One control of the conjugate checks the proper functioning of the enzymatic reaction.

•  One control of amplification which demonstrates a smooth proceeding for the PCR reaction.